1| Harvest exponentially growing cells 24 h before transfection by trypsinization and replate them at a density of 1 × 10 5 to 4 × 10 5 cells/cm 2 in 60-mm tissue culture dishes or in 12-well ...

During cell therapy development, some scientists are moving away from viral transfection and toward electroporation for introducing CRISPR systems and other nucleic acid payloads. As electroporation ...

Electroporation uses short electric pulses to create transient pores in a cell’s membrane, allowing researchers ... using the Neon NxT system that researchers can follow for optimal transfection ...