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During this week you will carry out restriction digestion of the genomic clone, in order to cut the insert (=the gene) from the plasmid DNA (=vector). Following separation of insert and vector by ...

This modified application of direct selection results in the production of clones in a sequence-ready format (in plasmid ... genomic rearrangements. Set up two reactions for digestion of genomic ...

"Sometimes, plasmids can integrate into genomic DNA, but this happens quite randomly ... cholerae bacteria as a model for studying the plasmid defense system known as DdmDE, consisting of proteins ...