First, negatively-charged RNA must cross hydrophobic cytoplasmic membranes at high concentrations during transfection. Additionally, exogenous RNA must not elicit a toxic immune response once inside ...

Developed in the late 1980s, liposomal transfection reagents work by enveloping the DNA or RNA and then fusing with the cell membrane to deposit the nucleic acid cargo inside. Roche Applied ...

9| To assay for transient expression of the introduced DNA, harvest the cells 1–6 d after transfection. Analyze RNA or DNA using hybridization; analyze the newly synthesized protein by ...